78th Annual Meeting of the APS Division of Fluid Dynamics (Nov 23 — 25, 2025)

V061: In Vivo Imaging Captures Cerebral Hemorrhage that Alters Cerebrospinal Fluid Flow in the Mouse Brain

Authors
  • Cooper Gray, University of Minnesota
  • Daehyun Kim , University of Minnesota
  • Dorothea Tse , University of Minnesota
  • Kyle Dieterle , University of Minnesota
  • Silas Simpson , University of Minnesota
  • Thomas Ruhl , University of Minnesota
  • Kyle G. Duff , University of Minnesota
  • Turki Alturki , University of Minnesota
  • Anika Volker , University of Minnesota
  • Jeffrey Tithof , University of Minnesota

In this video, fluorescent microspheres injected into the cerebrospinal fluid (CSF) of an alive, anesthetized mouse are imaged at high spatial and temporal resolutions in perivascular spaces (PVS) which are annular channels that surround blood vessels in the brain. To characterize the fluid mechanics, particle tracking velocimetry (PTV) was also applied to the dataset in postprocessing to analyze the CSF flow speeds, particle velocities, and particle trajectories. Incredibly, in this dataset a cerebral hemorrhage was also captured which drastically changed the nature of the CSF flow and offers insights into the interplay of CSF flow and blood flow in the brain. Our PTV analysis shows increased flow speeds near the site of the cerebral hemorrhage, and flow is diverted from the PVS into the location where the hemorrhage occurred.

This work is licensed under the Creative Commons Attribution-NonCommercial 4.0 International License. Any reuse must credit the author(s) and provide a link back to this page.